Pratheepa Rasiah

Pratheepa K. Rasiah, PhD — Research Assistant Professor, Vanderbilt University

Fluorescence micrograph of retinal pigment epithelial cells forming a cyan cobblestone mosaic
Confocal micrograph of a dense band of green glial fibres against a dark background
Confocal micrograph of microglia labelled in green scattered across dark brain tissue

Retinal pigment epithelium

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Pratheepa Rasiah

Pratheepa K. Rasiah

Research Assistant Professor
Department of Biomedical Engineering
Vanderbilt Biophotonics Center

About

Pratheepa K. Rasiah

With over 15 years of research experience, my work spans neuroscience, retinal biology, cellular and molecular biology, and translational biomedical research. Currently affiliated as a Research Assistant Professor in the Department of Biomedical Engineering at Vanderbilt University and a member of the Vanderbilt Biophotonics Center, I focus on developing innovative approaches to understand nervous system injury and repair.

Research expertise encompasses the design and execution of complex in vitro and in vivo studies, development of preclinical traumatic brain injury (TBI) models, establishment of primary neuronal and glial culture systems, and application of advanced optical imaging, microscopy, and molecular techniques. These efforts integrate engineering and biology to investigate mechanisms of neural injury and evaluate emerging therapeutic strategies.

A central focus of my research is the development of clinically relevant preclinical models that replicate real-world traumatic injuries. These models provide a platform for investigating the biological consequences of trauma and evaluating novel pharmaceuticals, biologics, and medical technologies aimed at improving recovery and informing the development of next-generation protective strategies.

Scientific interests also include retinal degeneration, neuroimmune responses following injury, optical neuromodulation, and the use of light-based technologies to probe and manipulate nervous system function. The overarching goal is to translate fundamental biological discoveries into meaningful advances for patient care.

Beyond research, I am committed to fostering collaborative and inclusive scientific environments through mentorship, interdisciplinary partnerships, and the advancement of diversity in STEM. As both a researcher and educator, I value creating opportunities that support the next generation of scientists.

Outside the laboratory, I enjoy reading and gardening—activities that inspire creativity, encourage curiosity, and provide balance beyond scientific research.

Core Competencies

  • Scientific Leadership
  • Program Management
  • Translational Research
  • Preclinical Development
  • Cross-functional Collaboration
  • Laboratory Operations
  • Neurotrauma
  • Regenerative Medicine
  • Biomedical Optics
  • Advanced Imaging
  • Scientific Communication

Experience

Research Experience

  1. 2022–Present

    Vanderbilt University

    Research Assistant Professor, Department of Biomedical Engineering | Vanderbilt Biophotonics Center

    Current research focuses on developing clinically relevant models of traumatic brain injury (TBI) to investigate the mechanisms of neural injury and evaluate emerging therapeutic strategies. Research integrates advanced optical imaging, nonlinear microscopy, primary neuronal and glial cell culture, and in vivo preclinical models to study neurotrauma, retinal injury, and neuroimmune responses. Ongoing work also explores optical stimulation technologies and regenerative approaches with the goal of translating fundamental discoveries into improved diagnostics and therapies.

  2. 2020–2021

    University of Tennessee Health Science Center

    Postdoctoral Research Fellow

    Investigated stem cell-based regenerative therapies for neurotrauma-associated retinal degeneration. Research examined how traumatic brain injury initiates progressive retinal damage through brain-eye signaling pathways and evaluated the therapeutic potential of mesenchymal stem cell-derived therapies to preserve retinal structure and function. The work combined neuroscience, ophthalmology, and regenerative medicine to address unmet clinical needs following neurotrauma.

  3. 2015–2017

    Duke University

    Postdoctoral Research Associate

    Studied ocular lens development and visual optics by investigating the molecular mechanisms governing lens maturation. Developed and characterized conditional knockout mouse models to define the role of Ankyrin G during lens development, demonstrating its essential function in regulating lens epithelial cell differentiation and maintaining normal lens architecture. This work provided new insights into the developmental biology underlying lens formation and visual function.

Service

Leadership & Professional Service

  • Faculty Senate Representative (Elected)

    School of Engineering, Vanderbilt University (2026–Present)

    Serve as an elected representative within Vanderbilt University's Faculty Senate, contributing to shared governance, institutional policy discussions, and initiatives that strengthen faculty engagement, research excellence, and academic advancement.

  • Co-Investigator, VA Merit Award

    2025–Present

    Provide scientific leadership for a multidisciplinary research program investigating traumatic brain injury–associated epilepsy and neurophysiological dysfunction. Responsibilities include experimental design, laboratory operations, protocol development, and coordination of collaborative research activities.

  • VUceptor Mentor

    Vanderbilt University (2023–Present)

    Mentor undergraduate students through Vanderbilt's VUceptor program, supporting their academic, personal, and professional development with an emphasis on resilience, leadership, scientific identity, and career preparation.

  • Early Career Editorial Board Member

    Journal of Advanced Photonics (2023–Present)

    Contribute to the editorial process by supporting peer review, promoting scientific rigor, and engaging with emerging developments in photonics and interdisciplinary biomedical research.

  • Scientific Advocacy

    National Alliance for Eye and Vision Research (NAEVR), Washington, D.C.

    Advocate for sustained federal investment in vision science and biomedical research through engagement with policymakers and participation in national initiatives that promote research innovation and public awareness.

Research timeline: from lens biology to neurotrauma — PhD lens biology (2011–2015), postdoctoral research at Duke (2015–2017), postdoctoral work at UTHSC (2020–2021), Research Assistant Professor at Vanderbilt (2022–present), and current focus on optical technologies and regenerative therapeutics.
Research timeline

Capabilities

Technical Expertise

My research integrates neuroscience, biomedical engineering, regenerative medicine, and advanced optical imaging to develop clinically relevant models of nervous system injury and evaluate novel therapeutic strategies. Technical expertise spans the complete preclinical research pipeline—from experimental design and animal models to molecular biology, advanced imaging, data analysis, and laboratory operations.

Preclinical Animal Models

  • Design and execution of rodent preclinical studies (mice and rats)
  • Traumatic brain injury models: Controlled Cortical Impact (CCI), blast injury, focused ultrasound injury, laser-induced shockwave injury, repetitive ocular blast injury
  • Ophthalmic disease models including retinal ischemia-reperfusion and diabetic retinopathy (STZ and Ins2AKITA)
  • Conditional knockout mouse generation, breeding strategies, colony management, and genotyping
  • Stereotaxic surgery, transcardial perfusion, vascular labeling, and in vivo functional vision assessments (ERG and OKN)
  • Drug administration via intravenous, intraperitoneal, subcutaneous, intranasal, oral gavage, intravitreal, and subretinal routes

Cell & Molecular Biology

  • Primary neuronal, astrocyte, epithelial, microglial, and stem cell culture
  • Co-culture systems, organotypic culture, and regenerative medicine applications
  • DNA/RNA isolation, cDNA synthesis, PCR, qPCR, Western blotting, immunoprecipitation, immunohistochemistry, and immunocytochemistry
  • Gene modulation using siRNA, shRNA, and CRISPR-based approaches
  • Real-time calcium imaging and Electric Cell-Substrate Impedance Sensing (ECIS)

Stem Cell & Regenerative Medicine

  • Mesenchymal stem cell culture and characterization
  • Secretome production, concentration, and functional evaluation
  • Extracellular vesicle and exosome isolation, purification, labeling, tracking, and in vivo delivery
  • Development of regenerative therapeutic strategies for neurotrauma and retinal degeneration

Optical Imaging & Microscopy

  • Confocal microscopy (Zeiss and Nikon platforms)
  • Light-sheet fluorescence microscopy and volumetric imaging
  • Tissue optical clearing and three-dimensional reconstruction
  • Nonlinear optical imaging and live tissue imaging
  • Brightfield, fluorescence, and phase-contrast microscopy
  • Quantitative image analysis and three-dimensional vascular mapping

Protein Biochemistry & Bioanalytical Methods

  • Protein purification, dialysis, chromatography, ultracentrifugation, and subcellular fractionation
  • Mass spectrometry sample preparation and protein characterization
  • ELISA, spectrophotometric assays, Raman spectroscopy, and biochemical analyses

Data Analysis & Scientific Software

  • ImageJ/Fiji
  • IMARIS
  • GraphPad Prism
  • MATLAB
  • Nikon NIS-Elements
  • Zeiss ZEN

Research Compliance & Laboratory Leadership

  • IACUC protocol development, amendments, and regulatory compliance
  • Animal colony oversight, procedural documentation, and experimental record keeping
  • Laboratory operations, inventory management, equipment oversight, and biosafety compliance
  • SOP development, workflow optimization, laboratory onboarding, and multidisciplinary team coordination

Contact

Get in touch

For research collaborations, speaking invitations, mentorship, or media inquiries, send a note and I'll get back to you.